(Running title)
Establishment and characterization of two UPSC cell lines
(Authors)
Tomohiro Iida1), Mieko Hamano2), Norio Yoshida1), Kyoko Yonamine1),
Kazuhiko Hayashi1), Kazushige Kiguchi1), Bunpei Ishizuka1),
Yukari Nishimura3), Tsutomu Arai3), Miwa Kawaguchi3), Hiroki Hata4),
Jun Watanabe5)
and Hiroyuki Kuramoto3)4).
1)Department of Obstetrics and Gynecology, St. Marianna University
School of Medicine, Kawasaki, Japan.
2)Tissue Culture Center,
4)Departments of Obstetrics and Gynecology and
5)Pathology, School of Medicine and
3)Department of Clinical Cytology, Graduate School of Medical
Sciences, Kitasato University. Sagamihara, Japan.
Address correspondence and reprint requests to:
Tomohiro Iida, M.D., Department of Obstetrics and Gynecology,
St. Marianna University School of Medicine,
2-16-1 Sugao, Miyamae-ku, Kawasaki 216-8511, Japan.
Abstract
Uterine papillary serous adenocarcinoma (UPSC) is an uncommon
histologic subtype of endometrial cancer that characteristically
behaves aggressively with a poor prognosis. We established two
novel cell lines derived from UPSC designated HEC-155 and HEC-180.
Both cell lines have been growing steady in monolayer cultures
for over ten years. Overexpression of p53, Ki67 and p27 was detected
in both cell lines by immunohistochemistry. Using DNA sequencing
technique, a point mutation of p53 was detected in exon 8, codon
286 in HEC-155 and in exon 6, codon 195 in HEC-180. These newly
established cell lines should be useful for investigating the
charasteristics of UPSC.
(Key Words) :uterine papillary serous carcinoma (UPSC); cell
line; p53 mutation