(Running Title)
Establishment of IM
(Authors)
Tomohiro IIDA1),Hiroyuki KURAMOTO2),Takao SHIMODA2),Mieko HAMANO2),Hiroki
HATA2),Kyoko YONAMINE1) and Kazuhiko HAYASHI1)
1)Department of Obstetrics and Gynecology St. Marianna University
School of Medicine, Yokohama city Seibu Hospital
2)Department of Obstetrics and Gynecology Kitasato University
School of Medicine
Abstract
A new cell line, designated IM has been established from operation
material derived from a woman with ovarian serous cystadenocarcinoma.
The population doubling time of the 35th passage IM cell was 28.8
hours. And it was successively subcultured 165 times in over 7
years, moreover still kept CA125 production. The nuclear DNA and
cell surface CA125 antigen were double stained by propidium iodide
and anti CA125 monoclonal antibody-FITC. Then the two color cytogram
obtained by flow cytometry was drawn up. For the most part of
CA125 positive cell retained G0+G1 of cell cycle, the lesser part
was in G2+M phese. The IC50 of IM cell incubated with cisplatin
at 37 or 41 for 1 hour were 6.9 Ęg/ml and 4.6 Ęg/ml. Then the
S phase rate was estimated by BrdU-propidium iodide double stain
method of flow cytometry. It was concluded that the inhibition
of DNA synthesis by cisplatin was increased with 41 low hyperthermia.
(Key Words) :Ovarian cancer cell line, CA125,Cisplatin, Flow
cytometry, Hyperthermia