(Running Title)
Establishment of SMOV-1
(Authors)
Tomohiro Iida (1), Kyoko Yonamine (1), Hisakiyo Takeuchi (1),
Kazuhiko Hayashi (1), Takashi Meguro (2), Toshihito Shinagawa
(3), and Akira Amemiya (4)
Department of Obstetrics and Gynecology (1) and the Research Laboratory
(2) of St. Marianna University Yokohama City Seibu Hospital.
1197-1 Yasashi, Asahi-ku Yokohama 241, Japan.
TEL (045)366-1111 FAX (045)366-1190
Department of the 1st. Division of Pathology (3) and the Department
of Obstetrics and Gynecology (4) of St. Marianna University School
of Medicine.
2-16-1 Sugao, Miyamae-ku, Kawasaki 216, Japan.
TEL (044)977-8111 FAX (044)977-2944
Abstract
Background
A novel cell line designated SMOV-1 was established from the ascitic
fluid of a woman with ovarian cancer. This report describes the
process of establishment and characterization of this cell line.
Methods
The case involved a woman who had undergone a surgical intervention
due to serious papillary adenocarcinoma of the ovary. She received
a PAC regimen after the surgery. However, as a result of malignant
pleural effusion during chemotherapy, she died 7 months after
surgery. Before surgery, ascitic fluid was removed from the patient
for cell culture after obtaining her consent. The cells were successfully
subcultured, and designated SMOV-1.
Results
The cell line gave a pavement stone pattern without contact inhibition.
SMOV-1 could also be transplantated into nude mice, and the tissue
showed reconstruction of the original tumor. The chromosome number
of SMOV-1 cells was 57, and the DNA index was 2.28. The population
doubling time of SMOV-1 cells was 61.4 hours. Cultured SMOV-1
cells were still capable of producing the tumor associated antigens
CA125, CA19-9, and STN.
Conclusion
We propose that this novel cell line is of possible use for the
investigation of drug resistance in ovarian cancer.
(Key Words) :ovarian cancer, in vitro, tumor marker, drug resistance